Developmental Toxicology Methods and Protocols

In Vitro Models in Developmental Toxicology.- Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments.- Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology.- Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model.- Micromass Methods for the Evaluation of Developmental Toxicants.- The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System.- Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology.- Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology.- Hepatotoxicity in Zebrafish Larvae.- Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology

Model.- The Chicken Embryo as a Model in Developmental Toxicology.- The
Xenopus tropicalis
Model for Studies of Developmental and Reproductive Toxicity.- Mouse Whole Embryo Culture.- Rat Whole Embryo Culture.- Rabbit Whole Embryo Culture.- Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology.- Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish.- Assessment of Histiotrophic Nutrition Using Fluorescent Probes.- Examining Gene Expression Patterns through Whole Mount In Situ Hybridization.- Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos.- Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection.- Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA.- Western Analysis of Breast Cancer 1 Protein (BRCA1).- Regulation and Control of AP-1 Binding Activity in Embryotoxicity.- Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR).- The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity.- Assessment of Gross Fetal Malformations:  The Modernized Wilson Technique and Skeletal Staining.