• Produktbild: Animal Cell Biotechnology
  • Produktbild: Animal Cell Biotechnology
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Animal Cell Biotechnology Methods and Protocols

Aus der Reihe Methods in Biotechnology
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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

05.04.2007

Abbildungen

w. 89 b&w and 3 col. Illustrationen

Herausgeber

Ralf Pörtner

Verlag

Humana Press

Seitenzahl

512

Maße (L/B/H)

24,1/16/3,7 cm

Gewicht

1054 g

Auflage

2nd edition 2007

Sprache

Englisch

ISBN

978-1-58829-660-3

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

05.04.2007

Abbildungen

w. 89 b&w and 3 col. Illustrationen

Herausgeber

Ralf Pörtner

Verlag

Humana Press

Seitenzahl

512

Maße (L/B/H)

24,1/16/3,7 cm

Gewicht

1054 g

Auflage

2nd edition 2007

Sprache

Englisch

ISBN

978-1-58829-660-3

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Animal Cell Biotechnology
  • Produktbild: Animal Cell Biotechnology
  • Animal Cell Biotechnology - Methods and Protocols

    Editor: Ralf Pörtner

    Contents

    Preface

    Contributors

    Part I. Basic techniques for establishing production cell lines

    1 Transduction Technologies
    David A. Fluri and Martin Fussenegger

    2 Cells for gene therapy and vector production
    C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten

    3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
    Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier

    4 DNA Fingerprinting and Characterisation of Animal Cell Lines
    Glyn N. Stacey, Ed Byrne and J. Ross Hawkins

    Part II. Basic cultivation techniques

    5 Microcarrier Cell culture Technology
    Gerald Blüml

    6 Cell Encapsulation
    Holger Hübner

    7 Tools for High-Throughput Medium and Process Optimisation
    Martin Jordan and Nigel Jenkins

    Part III. Cell characterisation and analysis

    8 Cell counting and viability measurements
    Michael Butler and Maureen Spearman

    9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry
    Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai

    10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
    André A. Neves and Kevin M. Brindle

    11 Methods for off-line analysis of nutrients and products in mammalian cell culture
    Heino Büntemeyer

    12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
    Derek Adams, Rashmi Korke, and Wei-Shou Hu

    13 Measurement of apoptosis in cell culture
    Adiba Ishaque1 and Mohamed Al-Rubeai

    14 Metabolic Flux Estimation in Mammalian Cell Cultures
    Chetan T. Goudar, Richard Biener, JamesM. Piret and Konstantin B. Konstantinov

    Part IV. Special cultivation techniques

    15 Disposable bioreactors for inoculum production and protein expression
    Regine Eibl and Dieter Eibl

    16 Hollow Fibre Cell Culture
    John M. Davis

    17 Cultivation of mammalian cells in fixed bed reactors
    Ralf Pörtner and Oscar B.J. Platas Barradas

    18 Configuration of Bioreactors
    Dirk E. Martens and Evert Jan van den End

    Part V. Downstream techniques

    19 Membrane filtration in animal cell culture
    Peter Czermak, Dirk Nehring and Ranil Wickramasinghe

    20 Chromatographic techniques in the downstream processing of (recombinant) proteins
    Ruth Freitag

    Part VI. Special applications

    21 Vaccine production - state of the art and future needs in upstream processing
    Yvonne Genzel and Udo Reichl

    22 Retrovirus production and characterization
    Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves

    23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production
    Sabine Geisse

    Index