Skin Tissue Engineering Methods and Protocols

Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture.- Isolation and Culture of Epidermal Melanocytes.- Initiating Separate Cultures of Keratinocytes and Melanocytes from a Single Biopsy.- Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor.- Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells.- Isolation and Culture of Human Dermal Fibroblasts.- Isolation and Culture of Human Dermal Microvascular Endothelial Cells Jennifer Bourland.- Isolation of stromal vascular fraction by fractionation of adipose tissue.- Engineering a multilayered skin equivalent: the importance of endogenous extracellular matrix and model flexibility for nuanced and accurate skin studies.- Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes.- Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models.- Engineering a pigmented skin equivalent that is responsive to external stimuli.- Bioprinting a tri-layered skin analogue.- Transdifferentiation of human dermis derived fibroblasts into iNeurons and cultivation in a collagen type 1 hydroge.- Deep proteome profiling of primary skin fibroblasts.- Characterization of human skin derived cells by Raman micro-spectroscopy.- Standard Preparation Protocol of Human Skin and Skin Substitute Samples for Transmission Electron Microscopy.- Methods for assessing scaffold vascularization with human endothelial cells.- Histological and histochemical methods for the evaluation of tissue engineered skin substitutes.- Pig Model to Test Tissue engineered Skin.- Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model.- Ex vivo human skin model to study immediate and early local responses to external stimuli.- A Method to Evaluate Skin and Skin Substitute Mechanical Properties Using a Suction Device.- GMP compliant production of therapeutic components of autologous adipose tissue.- Quality controls of autologous skin products according to European GMP guidelines.- Standardized outcome measures for the clinical application of tissue engineered products.