Historical DNA manipulation Overview.- Tools for efficient genome editing; ZFN, TALEN, and CRISPR.- Efficient generation of stable cell lines with inducible neuronal transgene expression using the piggyBac transposon system.- Modifying Bacterial Artificial Chromosomes for Extended Genome Modification.- Site specific editing of the genome in Pluripotent cells.- Targeting the AAVS1 site with transgene cassette by CRISPR/Cas9 with an inducible transgene cassette for the to neuronal differentiation of human pluripotent stem cells.- Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour.- CRISPR-on for endogenous activation of
SMARCA4
expression in bovine embryos.- CRISPR/Cas mutagenesis to generate novel traits in
Bactrocera

tryoni
for Sterile Insect Technique.- CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowfly.- Generation of gene drive mice for invasive pest population suppression.- Generation of a human deafness sheep model using the CRISPR/Cas system.- Targeted Gene Editing in Porcine Germ Cells.- Generating a Heat Tolerance Mouse Model.- Generation of pigs that produce single sex progeny.- Generation of Double-muscled Sheep and Goats by CRISPR/Cas9-mediated Knockout of the Myostatin Gene.- Regulatory and policy considerations around applications of genome editing in agriculture.