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Mycobacteria Protocols

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Beschreibung

This fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters...

Beschreibung

This fully updated edition explores the latest techniques to study the challenging, and at times dangerous, genus of bacteria known as mycobacteria with basic methods that are still required for mycobacteriology along with the newer or improved methods that have been developed. The volume features chapters on the basics of DNA isolation, protein isolation, and lipid isolation, as well as more sophisticated techniques for isolation of ribosomes, and continues with sections involving analyzing subcellular fractions, culture methods, sequencing technology,
in vitro
models, molecular methods, as well as drug discovery applications. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date,
Mycobacteria Protocols, Fourth Edition
serves as an ideal guide for those starting out with their mycobacteria research and also for those who have worked with it for decades.

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

08.07.2022

Abbildungen

XV, 736 p. 104 illus., 80 illus. in color.

Herausgeber Tanya Parish + weitere

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

08.07.2022

Abbildungen

XV, 736 p. 104 illus., 80 illus. in color.

Herausgeber
Verlag Springer Us
Seitenzahl

736

Maße (L/B/H)

25,4/17,8/4,1 cm

Gewicht

1388 g

Auflage

Fourth Edition 2021

Sprache

Englisch

ISBN

978-1-07-161462-4

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Mycobacteria Protocols
  • Produktbild: Mycobacteria Protocols

  • Culturing Mycobacteria.- DNA Isolation from Mycobacteria.- Extraction and Separation of Mycobacterial Proteins.- Lipid and Lipoarabinomannan Isolation and Characterization.- Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria.- Macrophage Infection Models for
    Mycobacterium tuberculosis
    .- Novel Single-Cell and High Throughput Microscopy Techniques to Monitor
    Dictyostelium discoideum
    -
    Mycobacterium marinum
    Infection Dynamics.- Single-Cell Analysis of Mycobacteria Using Microfluidics and Time-Lapse Microscopy.- Measuring Efflux and Permeability in Mycobacteria.- Drug Susceptibility Screening Using In Vitro Models of Hypoxic Non-Replicating Persistent Mycobacteria.- Flow Cytometry Analysis of Mycobacteria and Mycobacteria-Infected Immune Cells.- Electroporation of Mycobacteria.- Gene Switching and Essentiality Testing.- Oligo-Mediated Recombineering and Its Use for Making SNPs, Knockouts, Insertions, and Fusions in
    Mycobacterium tuberculosis
    .- Using Proteolytic Hypomorphs to Detect Small Molecule Mechanism of Action.- CRISPR Interference (CRISPRi) for Targeted Gene Silencing in Mycobacteria.- Exploiting Fluorescent Proteins to Understand
    Mycobacterium tuberculosis
    Biology.- Metabolic Labeling of Live Mycobacteria with Trehalose-Based Probes.- Identification and Characterization of Mycobacterial Species Using Whole Genome Sequences.- Whole Genome Sequencing of
    Mycobacterium tuberculosis
    Directly from Sputum Samples.- Experimental and Computational Workflow for RNA Sequencing in
    Mycobacterium tuberculosis
    : From Total RNA to Differentially Expressed Genes.- RNA Sequencing for Transcript 5′-End Mapping in Mycobacteria.- Methods for Proteomic Analyses of Mycobacteria.- Targeted Lipidomics of Mycobacterial Lipids and Glycolipids.- Metabolomics of
    Mycobacterium tuberculosis
    .- Determining Minimum Inhibitory Concentrations in Liquid Cultures or on Solid Medium.- A Multi-Stress Model for High Throughput Screening Against Non-Replicating
    Mycobacterium tuberculosis
    .- Antimicrobial Susceptibility Testing for
    Mycobacterium sps
    in High Throughput Format.- High-Content Analysis Monitoring Intracellular Trafficking and Replication of
    Mycobacterium tuberculosis
    Inside Host Cells.- Efficient Measurement of Drug Interactions with DiaMOND (Diagonal Measurement of N-Way Drug Interactions).- Drug Sensitivity Testing of
    Mycobacterium tuberculosis
    Growing in a Hollow Fiber Bioreactor.