Protein-Ligand Interactions Methods and Applications

Assessing and Improving Protein Sample Quality.- A Familiar Protein-Ligand Interaction Revisited with Multiple Methods.- Interactions of a Signal Transduction Protein Investigated by Fluorescence Stopped-Flow Kinetics.- Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins.- Isothermal Titration Calorimetry.- Measuring the K
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of Protein-Ligand Interactions Using Microscale Thermophoresis.- Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Preparing Functionalized Lipid Layers for the Study of Complex Protein-Ligand Interactions.- Indirect Detection of Ligand Binding by Thermal Melt Analysis.- The Use of Acoustic Mist Ionization Mass Spectrometry for High-throughput Screening.- Ligand Discovery – High Through-put Binding: Fluorescence Polarization (Anisotropy).- Fragment Screening by NMR.- A Quick Primer in Fluorescence-based Equilibrium and Pre-Steady State Methods for Determining Protein-Nucleotide Affinities.- Measurement of Nucleotide Hydrolysis using Fluorescent Biosensors for Phosphate.- Gel-based Analysis of Protein-Nucleic Acid Interactions.- Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome using SwitchSENSE.- Biolayer Interferometry: Protein-RNA Interactions.- Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip.- Characterization of Protein Nucleic Acid complexes by Size-Exclusion Chromatography Coupled with Light Scattering, Absorbance and Refractive Index Detectors.- Analytical Ultracentrifugation for Analysis of Protein Nucleic Acid Interactions.- Studying RNA-Protein Complexes Using X-ray Crystallography.- Flow Linear Dichroism of Protein-membrane Systems.- Probing Protein-Membrane Interactions and Dynamics Using Hydrogen-Deuterium eXchange Mass Spectrometry (HDX-MS).