Produktbild: Peroxisomes
Band 1595

Peroxisomes Methods and Protocols

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

20.07.2018

Abbildungen

XVII, 63 illus., 36 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Michael Schrader

Verlag

Springer Us

Seitenzahl

347

Maße (L/B/H)

25,4/17,8/2 cm

Gewicht

699 g

Auflage

Softcover reprint of the original 1st ed. 2017

Sprache

Englisch

ISBN

978-1-4939-8344-5

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

20.07.2018

Abbildungen

XVII, 63 illus., 36 illus. in color., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Michael Schrader

Verlag

Springer Us

Seitenzahl

347

Maße (L/B/H)

25,4/17,8/2 cm

Gewicht

699 g

Auflage

Softcover reprint of the original 1st ed. 2017

Sprache

Englisch

ISBN

978-1-4939-8344-5

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Peroxisomes
  • 1. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation

    Andreas Manner and Markus Islinger

     

    2. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes

    Miriam J. Schönenberger and Werner J. Kovacs

     

    3. Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays

    Tânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo

     

    4. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae

    Tobias Hansen, Anna Chan, Thomas Schröter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann

     

    5. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid

    Malu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp

     

    6. Analysis of Plasmalogen Synthesis in Cultured Cells

    Masanori Honsho and Yukio Fujiki<

     

    7. Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

    Janet Koster and Hans R. Waterham

     

    8. siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells

    Tina A. Schrader and Michael Schrader

     

    9. Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals

    Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms

     

    10. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3’-diaminobenzidine

    H. Dariush Fahimi

     

    11. Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins

    Karina Mildner and Dagmar Zeuschner

     

    12. Detection and Immunolabelling of Peroxisomal Proteins

    Tina Schrader, Markus Islinger, and Michael Schrader

     

    13. Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis

    Sofia C. Guimaraes, Sreedhar Kilaru, Michael Schrader, and Martin Schuster

     

    14. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes

    Celien Lismont, Paul A. Walton, and Marc Fransen

     

    15. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress

    Marc Fransen and Chantal Brees

     

    16. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed

    Luis F. Godinho, and Michael Schrader

     

    17. In Cellulo Approaches to Study Peroxisomal Protein Import – Yeast Immunofluorescence Microscopy

    Tobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann

     

    18. Blue Native Page: Applications to Study Peroxisome Biogenesis

    Kanji Okumoto, Shigehiko Tamura, and Yukio Fujiki

     

    19. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes

    Yuqiong Liu, Masanori Honsho, and Yukio Fujiki

     

    20. Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System

    Kanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki

     

    21. The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells

    Judith Buentzel, and Sven Thoms

     

    22. Detection of Ubiquitinated Peroxisomal Proteins in Yeast

    Natasha Danda and Chris Williams

     

    23. Assessing Pexophagy in Mammalian Cells

    Shun-ichi Yamashita and Yukio Fujiki

     

    24. Experimental Systems to Study Yeast Pexophagy

    Shun-ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, and Yukio Fujiki

     

    25. Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells

    Thomas Nury, Imen Helali, El Mostafa Karym, Meryam Debbabi, Flore Geillon, Catherine Gondcaille, Doriane Trompier, Amina Najid, Sébastien Terreau, Maryem Bezine, Amira Zarrouk, Anne Vejux, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Stéphane Savary, and Gérard Lizard

     

    26. Study of Peroxisomal Protein Phosphorylation by Functional Proteomics

    Andreas Schummer, Sven Fischer, Silke Oeljeklaus, and Bettina Warscheid

     

    27. Analysis of Peroxisomal β-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana Seedlings

    Björn Hielscher, Lennart Charton, Tabea Mettler-Altmann, and Nicole Linka

     

    28. Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made Easy

    Noa Dahan, Maya Schuldiner, and Einat Zalckvar

     

    29. Generation of Peroxisome-Deficient Somatic Animal Cell Mutants

    Kanji Okumoto and Yukio Fujiki

     

    30. Clinical and Laboratory Diagnosis of Peroxisomal Disorders

    Ronald J.A. Wanders, Femke C.C. Klouwer, Sacha Ferdinandusse, Hans R. Waterham, and Bwee Tien Poll-Thé