1. Journey of Human Gametes In Vitro: 1978 to 2010
Zeev Shoham
I. Setting Up and Running an IVF Laboratory
2. Building the Laboratory
Dean E. Morbeck and Marlena Duke
3. Air Quality Management
Johan Guns, Ronny Janssens, and Martine Vercammen
4. Organizational Aspects of the Laboratory in a Tertiary Care ART Center
Nancy L. Bossert and Christopher De Jonge
5. Quality Control Management
William R. Boone and H. Lee Higdon III
6. Daily, Weekly, and Regular Preparations for the IVF Laboratory
Michael A. Britt and Klaus E. Wiemer
7. Risk and Safety in the IVF Clinic
Peter Sjoblom and Julius Hreinsson
II. IVF Laboratory Equipment and Culture Systems
8. Essential Instruments and Disposable Supplies for an IVF Laboratory
Leslie Weikert, Christa Fralick, and Klaus E. Wiemer
9. Co2 and Low-O2 Incubators
Marius Meintjes
10. IVF Workstations
Nicolas Prados and Alex C. Varghese
11. Culture Media in IVF: Decisions for the Laboratory
Jason E. Swain and Thomas B. Pool
III. In Vitro Fertilization
12. Oocyte Denuding
Roberta Maggiulli, Filippo Ubaldi, and Laura Rienzi
13. Assessment of Oocyte Quality
Basak Balaban, Turgay Barut, and Bülent Urman
14. Polarization Microscopy
Markus Montag, Maria Köster, and Hans van der Ven
15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality
Dagan Wells
IV. Embryo Culture Methods
16. Short Culture: Day 1/Day 2/Day 3 Embryo Culture
Patrick Quinn
17. Extended Culture in IVF
David K. Gardner and Michelle Lane
18. In Vitro Maturation of Human Oocytes
Baris Ata, Jack Huang, and Ri-Cheng Chian
19. In Vivo Embryo Culture Device
Claude Ranoux
20. Microfluidics for Gamete Manipulation and Embryo Culture
Gary D. Smith, Charles Bormann, and Shuichi Takayama
V. Sperm Processing and Selection
21. Sperm Assessment: Traditional Approaches and Their Indicative Value
Margot Flint, Fanuel Lampiao, Ashok Agarwal, and Stefan S du Plessis
22. Sperm Assessment: Novel Approaches and Their Indicative Value
De Y. Liu, Harold Bourne, C. Garrett, G. N. Clarke, Shlomi Barak, and H. W. G. Baker
23. Sperm Processing for IVF
Ralf Henkel
24. PESA/TESA/TESE Sperm Processing
Sandro C. Esteves and Sidney Verza, Jr.
25. Processing Sperm Samples in HIV-Positive Patients
Thamara Viloria Samochin, Marcos Meseguer, Antonio Pellicer, José Remohí, and Nicolás Garrido
26. Intracytoplasmic Morphologically Selected Sperm Injection
P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher, Barbara Wirleitner, Martin Zintz, Bernard Lejeune, S. Vanderzwalmen, Nino Guy Cassuto, Mathias Zech, and Nicolas H. Zech
27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories
Gabor Huszar
28. Electrophoretic Sperm Separation
Steven Fleming and John Aitken
29. Magnetic Activated Cell Sorting of Human Spermatozoa
Enver Kerem Dirican
30. Poloscope-Based Sperm Selection
Luca Gianaroli, M. Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, and Anna P. Ferraretti
VI. Insemination Procedures
31. Intrauterine Insemination
Gautam N. Allahbadia and Rubina Merchant
32. Conventional IVF Insemination
Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, and Zsolt Peter Nagy
33. Intracytoplasmic Sperm Injection
Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, and Zev Rosenwaks
34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization
Dmitri Dozortsev and Mohammad Hossein Nasr-Esfahani
VII. Micromanipulators and Micromanipulation
35. Hydraulic Manipulators for ICSI
Hubert Joris
36. Research Instruments Micromanipulators
Steven Fleming and Catherine Pretty
37. Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators
Ehab Abu-Marar and Safa Al-Hasani
38. The Leica-IMSI System
Christiane Wittemer, Bruno Laborde, Frederic Ribay, and Stephane Viville
39. Automated Robotic Intracytoplasmic Sperm Injection
Zhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung, Navid Esfandiari, Robert F. Casper, and Yu Sun
40. Oocyte Treatment and Preparation for Microinjection
Thomas Ebner
41. Livestock Production Via Micromanipulation
Akira Onishi and Anthony C. F. Perry
VIII. Embryo Evaluation, Grading, and Assisted Hatching
42. Pronuclear Scoring in Human In Vitro Fertilization
Lynette Scott
43. Cumulative Morphological Assessment of Embryo Quality
Barry Behr and Aparna Hegde
44. Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability
D. Sakkas, L. Botros, M. Henson, K. Judge, and P. Roos
45. Oxygen Consumption as an Indicator of Oocyte and Embryo Viability
Ana S. Lopes
46. Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection
Samir Hamamah, Said Assou, Imène Boumela, Gisèle Ouandaogo, Hervé Dechaud, and John de Vos
47. Amino Acid Turnover as a Biomarker of Embryo Viability
Roger Sturmey, Christine Leary, Henry Leese, and Danielle Smith
48. Real-time Embryo Monitoring Devices for Embryo Selection
Gábor Vajta and Thorir Hardarson
49. Assisted Hatching in IVF
Itziar Belil and Anna Veiga
IX. Biopsy Procedures on Oocytes and Embryos
50. Polar Body Biopsy
Markus Montag, Maria Köster, K. van der Ven, and Hans van der Ven
51. Cleavage Stage Embryo Biopsy
Alan R. Thornhill
52. Embryo Biopsy for PGD: Current Perspective
Steven J. McArthur, Don Leigh, Maria Traversa, James Marshall, and Robert P. S. Jansen
53. Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects
Gary Harton and Santiago Munné
X. Cryopreservation
54. Sperm Cryopreservation
Fabio Firmbach Pasqualotto, Eleonora Bedin Pasqualotto, Edson Borges, Jr., and Ashok Agarwal
55. Slow Freezing of Oocytes
Giovanni Coticchio and Lucia De Santis
56. Vitrification: Research in Animal Models
Gábor Vajta
57. Oocyte Vitrification
Ana Cobo
58. Slow Freezing of Embryos
Liesl Nel-Themaat, Ching-Chien Chang, Thomas Elliott, Patricia Bernal, Graham Wright, and Zsolt Peter Nagy
59. Vitrification of Embryos
Juergen Liebermann, Joe Conaghan, Zsolt Peter Nagy, and Michael Tucker
60. Directional Freezing of Reproduction Cells, Tissues, and Organs
Amir Arav
61. Ovarian Tissue Cryopreservation
Jennifer L Kulp, J. Ryan Martin, and Pasquale Patrizio
XI. Embryo Transfer
62. Assessment of Uterine Receptivity
Hakan Cakmak and Hugh S. Taylor
63. Single Embryo Transfer
Thorir Hardarson and Matts Wikland
64. Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation
Ahmed M. Abou-Setta
65. Cumulus-Aided Embryo Transfer
Firuza R. Parikh, Nandkishor J. Naik, Dattatray J. Naik, and Dhanajaya Kulkarni
XII. Management and Regulation in the ART Laboratory
66. Data Management in the ART Laboratory: Requirements and Solutions
Timothy Brown and Bruce R. Gilbert
67. Regulation, Licensing, and Accreditation of the ART Laboratory
Doris Baker
68. Legislation in the United Kingdom
Rachel Cutting
69. Regulation, Licensing, and Accreditation of the ART Laboratory in Europe
Julius Hreinsson and Peter Sjoblom
70. Regulation, Licensing, and Accreditation of ART Laboratories in India
B. N. Chakravarty and Rita Modi
71. Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART
Kathryn J. Go, Jay C. Patel, and Rick Dietz
XIII. Special Topics
72. The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development
Jonathan Van Blerkom
73. Nuclear and Cytoplasmic Transfer: Human Applications and Concerns
Josef Fulka, Jr. and Helena Fulka
74. Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization
Heide Schatten, Vanesa Y. Rawe, and Qing-Yuan Sun
75. Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence
