• Produktbild: Insoluble Proteins
  • Produktbild: Insoluble Proteins
Band 1258

Insoluble Proteins Methods and Protocols

106,99 €

inkl. gesetzl. MwSt., Versandkostenfrei


Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Abbildungen

XVI, 425 p. 72 illus., 41 illus. in color.

Herausgeber

Elena García-Fruitós

Verlag

Springer Us

Seitenzahl

425

Maße (L/B/H)

25,4/17,8/2,4 cm

Gewicht

829 g

Auflage

Softcover reprint of the original 1st ed. 2015

Sprache

Englisch

ISBN

978-1-4939-4675-4

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Abbildungen

XVI, 425 p. 72 illus., 41 illus. in color.

Herausgeber

Elena García-Fruitós

Verlag

Springer Us

Seitenzahl

425

Maße (L/B/H)

25,4/17,8/2,4 cm

Gewicht

829 g

Auflage

Softcover reprint of the original 1st ed. 2015

Sprache

Englisch

ISBN

978-1-4939-4675-4

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Insoluble Proteins
  • Produktbild: Insoluble Proteins

  • General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins.- Overcoming the Solubility Problem in
    E. coli
    : Available Approaches for Recombinant Protein Production.- Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility.- Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification.- Beyond the Cytoplasm of
    Escherichia coli
    : Localizing Recombinant Proteins Where You Want Them.- Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates.- Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems.- Functional Expression of Plant Membrane Proteins in
    Lactococcus lactis.
    - High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of “Difficult-to-Express” Secretory Recombinant Proteins.- Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins.- Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins.- Recombinant Glycoprotein Production in Human Cell Lines.- Soluble Recombinant Protein Production in
    Pseudoaltermonas haloplanktis
    TAC125.- A Screening Methodology for Purifying Proteins with Aggregation Problems.- Solubilization and Refolding of Inclusion Body Proteins.- Bacterial Inclusion Body Purification.- Characterization of Intracellular Aggresomes by Fluorescent Microscopy.- Dialysis: A Characterization Method of Aggregation Tendency.- Applications of Mass Spectrometry to the Study of Protein Aggregation.- Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy.- Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR).- Methods for Characterization of Protein Aggregates.- Predicting the Solubility of Recombinant Proteins in
    Escherichia coli
    .- Insoluble ProteinApplications: The Use of Bacterial Inclusion Bodies as Biocatalysts.