Produktbild: Basic Applied Bioinformatics

Basic Applied Bioinformatics

152,99 €

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

13.11.2017

Verlag

John Wiley & Sons Inc

Seitenzahl

472

Maße (L/B/H)

24,6/17,5/3 cm

Gewicht

1179 g

Sprache

Englisch

ISBN

978-1-119-24433-2

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

13.11.2017

Verlag

John Wiley & Sons Inc

Seitenzahl

472

Maße (L/B/H)

24,6/17,5/3 cm

Gewicht

1179 g

Sprache

Englisch

ISBN

978-1-119-24433-2

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  • Produktbild: Basic Applied Bioinformatics
  • PREFACE, xi

    ACKNOWLEDGEMENTS, xiii

    LIST OF ABBREVIATIONS, xv

    SECTION I Molecular Sequences and Structures

    1 Retrieval of Sequence(s) from the NCBI Nucleotide Database, 3

    2 Retrieval of Protein Sequence from UniProtKB, 9

    3 Downloading Protein Structure, 15

    4 Visualizing Protein Structure, 19

    5 Sequence Format Conversion, 23

    6 Nucleotide Sequence Analysis Using Sequence Manipulation Suite (SMS), 31

    7 Detection of Restriction Enzyme Sites, 43

    SECTION II Sequence Alignment

    8 Dot Plot Analysis, 53

    9 Needleman-Wunsch Algorithm (Global Alignment), 59

    10 Smith-Waterman Algorithm (Local Alignment), 67

    11 Sequence Alignment Using Online Tools, 73

    SECTION III Basic Local Alignment Search Tools

    12 Basic Local Alignment Search Tool for Nucleotide (BLASTn), 81

    13 Basic Local Alignment Search Tool for Amino Acid Sequences (BLASTp), 91

    14 BLASTx, 103

    15 tBLASTn, 109

    16 tBLASTx, 113

    SECTION IV Primer Designing and Quality Checking

    17 Primer Designing - Basics, 121

    18 Designing PCR Primers Using the Primer3 Online Tool, 125

    19 Quality Checking of the Designed Primers, 139

    20 Primer Designing for SYBR Green Chemistry of qPCR, 147

    SECTION V Molecular Phylogenetics

    21 Construction of Phylogenetic Tree: Unweighted?]Pair Group Method with Arithmetic Mean (UPGMA), 151

    22 Construction of Phylogenetic Tree: Fitch Margoliash (FM) Algorithm, 159

    23 Construction of Phylogenetic Tree: Neighbor?]Joining Method, 165

    24 Construction of Phylogenetic Tree: Maximum Parsimony Method, 175

    25 Construction of Phylogenetic Tree: Minimum Evolution Method, 183

    26 Construction of Phylogenetic Tree Using MEGA7, 187

    27 Interpretation of Phylogenetic Trees, 197

    SECTION VI Protein Structure Prediction

    28 Prediction of Secondary Structure of Protein, 211

    29 Prediction of Tertiary Structure of Protein: Sequence Homology, 217

    30 Protein Structure Prediction Using Threading Method, 223

    31 Prediction of Tertiary Structure of Protein: Ab Initio Approach, 229

    32 Validation of Predicted Tertiary Structure of Protein, 235

    SECTION VII Molecular Docking and Binding Site Prediction

    33 Prediction of Transcription Binding Sites, 243

    34 Prediction of Translation Initiation Sites, 251

    35 Molecular Docking, 257

    SECTION VIII Genome Annotation

    36 Genome Annotation in Prokaryotes, 265

    37 Genome Annotation in Eukaryotes, 269

    SECTION IX Advanced Biocomputational Analyses

    38 Concepts of Real?]Time PCR Data Analysis, 275

    39 Overview of Microarray Data Analysis, 283

    40 Single Nucleotide Polymorphism (SNP) Mining Tools, 289

    41 In Silico Mining of Simple Sequence Repeats (SSR) Markers, 299

    42 Basics of RNA?]Seq Data Analysis, 305

    43 Functional Annotation of Common Differentially Expressed Genes, 313

    44 Identification of Differentially Expressed Genes (DEGs), 325

    45 Estimating MicroRNA Expression Using the miRDeep2 Tool, 357

    46 miRNA Target Prediction, 365

    Appendices

    Appendix A: Usage of Internet for Bioinformatics, 377

    Appendix B: Important Web Resources for Bioinformatics Databases and Tools, 381

    Appendix C: NCBI Database: A Brief Account, 389

    Appendix D: EMBL Databases and Tools: An Overview, 395

    Appendix E: Basics of Molecular Phylogeny, 403

    Appendix F: Evolutionary Models of Molecular Phylogeny, 411

    GLOSSARY, 415

    REFERENCES, 423

    WEBLIOGRAPHY, 431

    INDEX, 435