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Phytoplasma Methods and Protocols

Aus der Reihe Methods in Molecular Biology
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142,99 € UVP 164,99 €

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

18.09.2012

Herausgeber

Matt Dickinson + weitere

Verlag

Humana Press

Seitenzahl

421

Maße (L/B/H)

26/18,3/2,9 cm

Gewicht

963 g

Auflage

2013

Sprache

Englisch

ISBN

978-1-62703-088-5

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

18.09.2012

Herausgeber

Verlag

Humana Press

Seitenzahl

421

Maße (L/B/H)

26/18,3/2,9 cm

Gewicht

963 g

Auflage

2013

Sprache

Englisch

ISBN

978-1-62703-088-5

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Phytoplasma
  • Produktbild: Phytoplasma

  • 1. The Phytoplasmas - An Introduction

                    Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts

     

    2. Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus

                    Jennifer Hodgetts, David Crossley, and Matt Dickinson

     

    3. Micropropagation and Maintenance of Phytoplasmas in Tissue Culture

                    Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo   

     

    4. Dodder Transmission of Phytoplasmas

    Jaroslava Přibylová and Josef Špak

     

    5. Insect Maintenance and Transmission

                    Heather Kingdom

     

    6. Capturing Insect Vectors of Phytoplasmas

                    Phyllis Weintraub and Jürgen Gross

     

    7. Insect Vector Transmission Assays

                    Domenico Bosco and Rosemarie Tedeschi

     

    8. Molecular Identification of Phytoplasma Vector Species

                    Sabrina Bertin and Domenico Bosco       

     

    9. Dienes’ Staining and Light Microscopy for Phytoplasma Visualization

                    Rita Musetti      

     

    10. DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas

                    Nancy S. Andrade and Nolberto S. Arismendi

                   

    11. Visualisation of Phytoplasmas using Electron Microscopy

                    B. Jean Devonshire

     

    12. Automated DNA Extraction for Large Numbers of Plant Samples

                    Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia

     

    13. DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts

                    Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick

     

    14. Nested PCR and RFLP Analysis Based on the 16S rRNA Gene

                    Bojan Duduk, Samantha Paltrinieri, Ing Ming Lee, and Assunta Bertaccini

     

    15. PCR and RFLP Analyses Based on the Ribosomal Protein Operon

                    Marta Martini and Ing-Ming Lee

     

    16. Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

                    Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová, and Milan Navrátil

     

    17. PCR Analysis of Phytoplasmas Based on the secA Gene

                    Matt Dickinson and Jennifer Hodgetts

     

    18. Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains

                    Martina Šeruga Musić and Dijana Škorić

     

    19. Microarrays for Universal Detection and Identification of Phytoplasmas

                    Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccine

     

    20. T-RFLP for Detection and Identification of Phytoplasmas in Plants

                    Jennifer Hodgetts and Matt Dickinson

     

    21. Real-Time PCR for Universal Phytoplasma Detection and Quantification

                    Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen

     

    22. A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA

                    Nataša Mehle, Nina Prezelj, Matjaž Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia

     

    23. Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group

                    Nataša Mehle, Petra Nikolić, Kristina Gruden, Maja Ravnikar, and Marina Dermastia

     

    24. Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

                    Paola Margaria and Sabrina Palmano

     

    25. In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP)

                    Jenny Tomlinson

     

    26. DNA Bar-Coding for Phytoplasma Identification

                    Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, and Mogens Nicolaisen

     

    27. Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison

                    Hiromi Nishida

     

    28. The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment

                    Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, and Robert E. Davis

     

    29. Phytoplasma Proteomic Analysis

                    Xianling Ji and Yingping Gai

     

    30. Preparation of Phytoplasma Membrane Recombinant Proteins

                    Luciana Galetto, Majid Siampour, and Cristina Marzachì

     

    31. Phytoplasma Plasmid DNA Extraction

                    Mark T. Andersen and Lia W. Liefting

     

    32. Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA

                    Lucy T.T. Tran-Nguyen and Bernd Schneider

     

    33. Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants

                    Carmine Marcone

     

    34. Mapping the Phytoplasma Chromosome

                    Sylvie Malembic-Maher and Patricia Carle