Part I: Mechanical Detectors 1. A Set of Piezoelectric Biosensors Using Cholinesterases Carsten Teller, Jan Halámek, Alexander Makower and Frieder W. Scheller 2. Piezoelectric Biosensors for Aptamer–Protein Interaction Sara Tombelli, Alessandra Bini, Maria Minunni and Marco Mascini 3. Piezoelectric Quartz Crystal Resonators Appliedfor Immunosensing and Affinity Interaction Studies Petr Skládal 4. Biosensors Based on Cantilevers Mar Álvarez, Laura G. Carrascosa, Kiril Zinoviev, Jose A. Plaza and Laura M. Lechuga 5. Piezoelectric-Excited Millimeter-Sized Cantilever (PEMC) Biosensors Raj Mutharasan Part II: Electrochemical Detectors 6. Preparation of Screen-Printed Electrochemical Immunosensors for Estradiol, and Their Application in Biological Fluids Roy M. Pemberton and John P. Hart 7. Electrochemical DNA Biosensors: Protocols for Intercalator-based Detection of Hybridization in Solution and at the Surface Kagan Kerman, Mun'delanji Vestergaard and Eiichi Tamiya 8. Electrochemical Biosensor Technology: Application to Pesticide Detection Ilaria Palchetti, Serena Laschi and Marco Mascini 9. Electrochemical Detection of DNA Hybridization Using Micro and Nanoparticles María Teresa Castañeda, Salvador Alegret and Arben Merkoçi 10. Electrochemical Immunosensing Using Micro and Nanoparticles Alfredo de la Escosura, Adriano Ambrosi, Salvador Alegret and Arben Merkoçi 11. Methods for the Preparation of Electrochemical Composite Biosensors Based on Gold Nanoparticles A. González-Cortés, P. Yáñez-Sedeño and J.M. Pingarrón Part III: Lateral Flow 12.Immunochromatographic Lateral Flow Strip Tests Gaiping Zhang, Junqing Guo and Xuannian Wang 13. Liposome-enhanced Lateral-flow Assays for the Sandwich-Hybridization Detection of RNA Katie A. Edwards and Antje J. Baeumner 14. Rapid Prototyping of Lateral Flow Assays Alexander Volkov, Michael Mauk, Paul Corstjens and R. Sam Niedbala 15. Lateral Flow Colloidal Gold-based Immunoassay for Pesticide Shuo Wang, Can Zhang and Yan Zhang Part IV: Ligands 16. Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen Juan E. Diaz, Li-Mei C. Yang, Jorge A. Lamboy, Reginald M. Penner and Gregory A. Weiss 17. In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents Jessica R. Newton and Susan L. Deutscher 18. Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands Michael J. McGuire, Shunzi Li, and Kathlynn C. Brown 19. Biosensor Detection Systems: Engineering Stable, High-Affinity Bioreceptors by Yeast Surface Display Sarah A. Richman, David M. Kranz and Jennifer D. Stone 20. Antibody Affinity Optimization Using Yeast Cell Surface Display Robert W. Siegel 21. Using RNA Aptamers and the Proximity Ligation Assay (PLA) for the Detection of Cell Surface Antigens Supriya S. Pai and Andrew D. Ellington 22. In Vitro Selection of Protein-binding DNA Aptamers as Ligands for Biosensing Applications Naveen K Navani, Wing Ki Mok and Yingfu Li Part V: Protein and DNA Preparation 23. Immobilization of Biomolecules onto Silica and Silica-based Surfaces for Use in Planar Array Biosensors Lisa C. Shriver-Lake, Paul T. Char