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Protein Sequencing Protocols

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.11.2010

Herausgeber

Bryan John Smith

Verlag

Humana Press

Seitenzahl

493

Maße (L/B/H)

22,9/15,2/3,2 cm

Gewicht

728 g

Auflage

2nd Edition.

Sprache

Englisch

ISBN

978-1-61737-284-1

Beschreibung

Rezension

"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives valuable background and insight to the various techniques associated with protein sequencing, which are often used by those providing services from within a core facility to the wider population of life science researchers." -Proteomics

"A positive feature of this book is that the fact that minute details, which are essential for successful implementation of an analytical method are provided. This book can serve as a useful reference for laboratories which mainly utilize chemical methods for protein analysis." -Pharmaceutical Research

From Reviews of the First Edition...

"...full of valuable information..." -Biochemical Education

"...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters

"...an extremely useful compendium of protein analysis protocols..." -Nahrung-Food

"...well organized and accessible..." -Journal of the American Chemical Society

"The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.11.2010

Herausgeber

Bryan John Smith

Verlag

Humana Press

Seitenzahl

493

Maße (L/B/H)

22,9/15,2/3,2 cm

Gewicht

728 g

Auflage

2nd Edition.

Sprache

Englisch

ISBN

978-1-61737-284-1

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Protein Sequencing Protocols
  • Produktbild: Protein Sequencing Protocols
  • Strategies for Handling Polypeptides on a Micro-Scale
    Bryan John Smith and Paul Tempst

    SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing
    Bryan John Smith

    Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization
    Michael J. Dunn

    Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization
    Michael J. Dunn

    Enzymatic Cleavage of Proteins
    Bryan John Smith

    Chemical Cleavage of Polypeptides
    Bryan John Smith

    Enzymatic Preparation and Isolation of Glycopeptides
    David J. Harvey

    Analytical and Micropreparative Capillary Electrophoresis of Peptides
    Alan J. Smith

    High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues
    Christoph W. Turck

    Hydrolysis of Samples for Amino Acid Analysis
    Ian Davidson

    Amino Acid Analysis: Precolumn Derivatization Methods
    G. Brent Irvine and Ian Davidson

    Post Column Amino Acid Analysis
    Alan J. Smith

    Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples
    Steven A. Cohen

    Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection
    Petr Jandik, Jun Cheng, and Nebojsa Avdalovic

    d-Amino Acid Analysis
    Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa

    Validation of Amino Acid Analysis Methods
    Andrew J. Reason

    Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins
    Fiona M. Greer and Howard R. Morris

    Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry
    Darryl J. C. Pappin

    Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes
    Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, and Andrej Shevchenko

    Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis
    David M. Schieltz and John R. Yates, III

    Identification of PTH-Amino Acids by HPLC
    Gregory A. Grant and Mark W. Crankshaw

    Protein Sequencer Maintenance and Troubleshooting
    Bryan Dunbar

    N-Terminal Protein Sequencing for Special Applications
    Philip J. Jackson

    Identification of Phosphorylation Sites by Edman Degradation
    Laurey Steinke and Richard G. Cook

    Validation of Protein Sequencing in a Regulated Laboratory
    Philip J. Jackson and Neil Dodsworth

    Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method
    David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham

    Chemical Modifications of Proteins as an Aid to Sequence Analysis
    Alex F. Carne

    Deblocking of N-Terminally Modified Proteins
    Hisashi Hirano and Roza M. Kamp

    Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid
    Jacek Mozdzanowski

    Identification of Sites of Protein Glycosylation
    David J. Harvey

    Analysis of Sites of Protein Phosphorylation
    Alastair Aitken and Michele Learmonth

    Quantitation and Location of Disulfide Bonds in Proteins
    Alastair Aitken and Michele Learmonth

    Getting the Most from Your Protein Sequence
    Richard R. Copley and Robert B. Russell

    Appendix 1: Letter Codes, Structures, Masses, and Derivatives of Amino Acids
    James P. Turner

    Appendix 2: Protein Consensus Sequence Motifs
    Alastair Aitken

    Index