Culture and Phenotyping of Chondrocytes in Primary Culture
Sylvie Thirion and Francis Berenbaum

Culture of Chondrocytes in Alginate Beads
Frédéric De Ceuninck, Christophe Lesur, Philippe Pastoureau, Audrey Caliez, and Massimo Sabatini

Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines
Mary B. Goldring

Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function
Mary B. Goldring

Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype
Luis A. Solchaga, Jean F. Welter, Donald P. Lennon, and Arnold I. Caplan

Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR
Gaëlle Rolland-Valognes

Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies
Audrey McAlinden, Jochen Haag, Brigitte Bau, Pia M. Gebhard, and Thomas Aigner

RNA Extraction From Cartilage
Frédéric Mallein-Gerin and Jérôme Gouttenoire

Gene Expression Analysis in Cartilage by In Situ Hybridization
Frédéric Mallein-Gerin and Jérôme Gouttenoire

Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis
Thomas Aigner, Joachim Saas, Alexander Zien, Ralf Zimmer, Pia M. Gebhard, and Thomas Knorr

High-Efficiency Nonviral Transfection of Primary Chondrocytes
Jean F. Welter, Luis A. Solchaga, and Matthew C. Stewart

In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors
Jean-Noel Gouze, Martin J. Stoddart, Elvire Gouze, Glyn D. Palmer, Steven C. Ghivizzani, Alan J. Grodzinsky, and Christopher H. Evans

Changes of Chondrocyte Metabolism In Vitro: An Approach by Proteomic Analysis
Anne-Marie Freyria and Michel Becchi

Analysis of ChondrocyteFunctional Markers and Pericellular Matrix Components by Flow Cytometry
Gust Verbruggen, Jun Wang, Lai Wang, Dirk Elewaut, and Eric M. Veys

A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
Frédéric De Ceuninck and Audrey Caliez

Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants
Christophe Lesur and Massimo Sabatini

Production of Antibodies Against Degradative Neoepitopes in Aggrecan
John S. Mort and Peter J. Roughley

Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures
R. Clark Billinghurst, Fackson Mwale, Anthony Hollander, Mirela Ionescu, and A. Robin Poole

Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes
Darryl D. D'Lima, Klaus Kuhn, and Martin K. Lotz

Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes
Jang-Soo Chun

Mechanical Loading of Chondrocytes Embedded in 3D Constructs: In Vitro Methods for Assessment of Morphological and Metabolic Response to Compressive Strain
David A. Lee and Martin M. Knight

In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage
Kelvin W. Li, Travis J. Klein, Kanika Chawla, Gayle E. Nugent, Won C. Bae, and Robert L. Sah

Index