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Produktbild: Cancer Cell Culture
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Cancer Cell Culture Methods and Protocols

Aus der Reihe Methods in Molecular Medicine
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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

November 2010

Abbildungen

98 schwarzweisse Abbildungen

Herausgeber

Simon P. Langdon

Verlag

Springer

Seitenzahl

368

Maße (L/B/H)

22,9/15,2/2,1 cm

Gewicht

526 g

Auflage

2004.

Sprache

Englisch

ISBN

978-1-61737-343-5

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

November 2010

Abbildungen

98 schwarzweisse Abbildungen

Herausgeber

Simon P. Langdon

Verlag

Springer

Seitenzahl

368

Maße (L/B/H)

22,9/15,2/2,1 cm

Gewicht

526 g

Auflage

2004.

Sprache

Englisch

ISBN

978-1-61737-343-5

Herstelleradresse

Springer Heidelberg
Tiergartenstr. 17
69121 Heidelberg
DE
buchhandel-buch@springer.com

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  • Produktbild: Cancer Cell Culture
  • Part I. Introduction to Cancer Cell Culture

    Basic Principles of Cancer Cell Culture
    Simon P. Langdon

    Essential Techniques of Cancer Cell Culture
    Kenneth G. Macleod and Simon P. Langdon

    Part II. Characterization and Authentication

    Characterization and Authentication of Cancer Cell Lines: An Overview
    Simon P. Langdon

    Authentication of Cancer Cell Lines by DNA Fingerprinting
    Wilhelm G. Dirks and Hans G. Drexler

    Cytogenetic Characterization of Tumor Cell Lines
    Roderick A. F. MacLeod and Hans G. Drexler

    Part III. Isolation and Culture of Specialized Cell Types

    Isolation and Culture of Colon Cancer Cell Lines
    Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park

    Isolation and Culture of Melanoma Cell Lines
    Adam I. Riker

    Isolation and Culture of Human Brain Tumor Cells
    German G. Gomez and Carol A. Kruse

    Isolation and Culture of Renal Cancer Cell Lines
    Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park

    Isolation and Culture of Prostate Cancer Cell Lines
    Nora M. Navone, Matilde Olive, and Patricia Troncoso

    Isolation and Culture of Ovarian Cancer Cell Lines
    Simon P. Langdon

    Isolation and Culture of Leukemia Cell Lines
    Hans G. Drexler

    Part IV. Functional Assays in Cell Culture

    Cell Sensitivity Assays: Clonogenic Assay
    Jane A. Plumb

    Cell Sensitivity Assays: The MTT Assay
    Jane A. Plumb

    PARP Cleavage as a Means of Assessing Apoptosis
    Peter Mullen

    Detection of Apoptosis by the TUNEL Assay
    John Lawry

    Apoptosis Measurement by Annexin V Staining
    Eric Miller

    Cell Adhesion Assays
    William N. Scott

    Radial Monolayer Cell Migration Assay
    Michael E. Berens and Christian Beaudry

    Invasion and Motility Assays
    William N. Scott

    Identification of Senescence in Cancer Cells
    Lesley J. Jardine

    Angiogenesis Assays
    William N. Scott

    Flow Cytometric DNA Analysis of Human Cancer Cell Lines
    Peter Mullen

    Part V. Modification of Cancer Cells

    DNA-Mediated Gene Transfer
    Amanda J. M. O'Donnell

    Development of Drug-Resistant Models
    Helen M. Coley

    Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
    Norman J. Maitland, Catherine A. Macintosh, Christian Schmitz, and Shona H. Lang

    The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts
    Peter Mullen

    Part VI. Coculture Systems

    Coculture of Prostate Cancer Cells
    Shona H. Lang and Norman J. Maitland

    Coculture of Ovarian Cells Using Porous Tissue Culture Inserts
    Katherine E. Snow and Simon P. Langdon

    Part VII. Cell Line Contamination

    Cell Culture Contamination: An Overview
    Simon P. Langdon

    Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
    Cord C. Uphoff and Hans G. Drexler

    Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
    Cord C. Uphoff and Hans G. Drexler

    Appendix 1: Formulations of Commonly Used Cell Culture Media

    Appendix 2: Human Cancer Cell Lines Available from the ATCC and DSMZ Cell Banks (Combined List)

    Index