1. Methods and Biology.- I. Isolated hepatocytes: forty years on.- 2. Isolation and culture of human hepatocytes.- 3. Isolation and culture of porcine hepatocytes.- 4. Preparation and properties of isolated hepatocytes from sheep.- 5. Elasmobranch hepatocytes.- 6. Isolation and cultivation of teleost hepatocytes.- 7. Conditions for growth of hepatocytes in culture.- 8. Engineering of a miniature 3-D hepatic cell plate culture model.- 9. Freezing and hypothermic storage of porcine hepatocytes.- 10. Storage of isolated hepatocytes.- 11. Hepatocyte swelling: techniques and effects on metabolism.- 12. Permeabilisation of hepatocytes with ?-toxin.- 13. Perifused immobilised hepatocytes for metabolic studies.- 14. Small hepatocytes in primary cultures.- 2. Reviews of Hepatocyte Biology.- 15. Mechanisms of hepatocyte growth regulation by hormones and growth factors.- 16. Proliferative response and metabolic effects of growth factors in human hepatocytes.- 17. Growth control and cell cycle progression in cultured hepatocytes.- 18. Mechanisms of active cell death in isolated hepatocytes.- 19. The protective role of nitric oxide in hepatocytes during responses to inflammatory mediators and induction of apoptosis.- 20. Short-term regulation by insulin of glucose metabolism in isolated and cultured hepatocytes.- 21. Studies on mitochondrial-cytoplasmic interactions in isolated hepatocytes from fasted rats.- 3. Applications: Pharmacology and Toxicology.- 22. The importance of hepatocytes in drug metabolism studies: an industrial perspective.- 23. Human hepatocytes as an experimental system for the evaluation of xenobiotics.- 24. Constitutive expression of cytochromes P450 in rat hepatocyte culture.- 25. Optimising conditions for induction of cytochrome P450 in primary hepatocytecultures.- 26. The use of isolated hepatocytes to study the mechanisms of action of environmental contaminants.- 27. The use of isolated hepatocytes for the study of xenobiotic hepatotoxicity.- 4. Therapeutic Applications.- 28. Human hepatocyte transplantation: biology and therapy.- 29. Ex vivo hepatocyte-directed gene therapy.- 30. A review of bioartificial liver development from an artificial organ engineering perspective.- 31. Development and testing of a bioartificial liver.- 32. The Berlin extracorporeal liver support system.- 33. The bioartificial liver of the Academic Medical Center at Amsterdam.- 34. Performance of a flat membrane bioreactor utilizing porcine hepatocytes cultured in an extracellular matrix.