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Produktbild: Introduction to Modern Liquid Chromatography

Introduction to Modern Liquid Chromatography

186,99 €

inkl. gesetzl. MwSt., Versandkostenfrei


Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

01.12.2009

Verlag

John Wiley & Sons

Seitenzahl

960

Maße (L/B/H)

26/18,3/5,5 cm

Gewicht

1956 g

Auflage

3rd edition

Sprache

Englisch

ISBN

978-0-470-16754-0

Beschreibung

Rezension

"In summary, I would strongly recommend this book for anyone working with LC and LC/MS. It is a finely crafted introduction that adequately covers nearly every aspect of the science. The emphasis on basic principles and practical aspects ensures that it will be a useful reference for many years to come." (J Am Soc Mass Spectrom, 2011)
 
"This third edition is highly cross-referenced, so as to allow the reader to follow up on topics of special interest, or to clarify questions that may arise during reading. The third edition of Introduction to Modern Liquid Chromatography will continue to be the HPLC reference book for thousands of readers, either experienced workers who may wish to explore topics of his/her choice, or find an answer to specific problems, or beginners who would like to understand and know the possibilities offered by the
technique." (Chemistry Journals, 11 April 2011)
 
"This classic text on liquid chromatography has been thoroughly updated through the addition of information on modern instrumentation, columns, and troubleshooting. It is a valuable resource for practicing chromatographers at all levels.." (Anal Bioanal Chem, 2011)
 
"In summary, I would strongly recommend this book for anyone working with LC and LC/MS. It is a finely crafted introduction that adequately covers nearly every aspect of the science. The emphasis on basic principles and practical aspects ensures that it will be a useful reference for any years to come." (American Society for Mass Spectrometry, 21 Januay 2011)
 
"The text is illustrated with many figures and tables originating from authors' own work or taken from the literature . . . both groups of readers will find in this book plenty of information and inspiration." (Journal of Separation Science, 1 June 2010)
 
"Following their highly successful second edition (1979), which appeared more than 30 years ago, Snyder (LC Resources) and Kirkland (Advanced Materials Technology) have teamed with Dolan (LC Resources) and additional collaborators to provide an excellent update of their earlier work." (CHOICE, July 2010)
 
"It is difficult in these times to ever call a scientific book a "bargain", but this truly is. The wealth of information contained in these almost 1000 pages is invaluable. The book is comprehensive, eminently readable and approachable, and highly useful for both the skilled chromatographer and those new to the technique. Everyone using a liquid chromatographic instrument would benefit from owning a copy." (JACS, 2010)
 
"It's current, clearly-indexed, well-referenced, and comprehensive. The inclusion of John Dolan as author has significantly expanded the sections on troubleshooting, and additional expert contributors have enhanced the coverage of the specialist chapters." - Bruce Hamilton posted on the Chromatography Forum

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

01.12.2009

Verlag

John Wiley & Sons

Seitenzahl

960

Maße (L/B/H)

26/18,3/5,5 cm

Gewicht

1956 g

Auflage

3rd edition

Sprache

Englisch

ISBN

978-0-470-16754-0

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Introduction to Modern Liquid Chromatography
  • Preface xxxi

    Glossary of Symbols and Abbreviations xxxv

    1 Introduction 1

    1.1 Background Information 2

    1.2 A Short History of HPLC 6

    1.3 Some Alternatives to HPLC 8

    1.4 Other Sources of HPLC Information 12

    References 15

    2 Basic Concepts and the Control of Separation 19

    2.1 Introduction 20

    2.2 The Chromatographic Process 20

    2.3 Retention 24

    2.4 Peak Width and the Column Plate Number N 35

    2.5 Resolution and Method Development 54

    2.6 Sample Size Effects 69

    2.7 Related Topics 74

    References 83

    3 Equipment 87

    3.1 Introduction 88

    3.2 Reservoirs and Solvent Filtration 89

    3.3 Mobile-Phase Degassing 92

    3.4 Tubing and Fittings 96

    3.5 Pumping Systems 104

    3.6 Autosamplers 113

    3.7 Column Ovens 125

    3.8 Data Systems 127

    3.9 Extra-Column Effects 131

    3.10 Maintenance 131

    References 144

    4 Detection 147

    4.1 Introduction 148

    4.2 Detector Characteristics 149

    4.3 Introduction to Individual Detectors 160

    4.4 UV-Visible Detectors 160

    4.5 Fluorescence Detectors 167

    4.6 Electrochemical (Amperometric) Detectors 170

    4.7 Radioactivity Detectors 172

    4.8 Conductivity Detectors 174

    4.9 Chemiluminescent Nitrogen Detector 174

    4.10 Chiral Detectors 175

    4.11 Refractive Index Detectors 177

    4.12 Light-Scattering Detectors 180

    4.13 Corona-Discharge Detector (CAD) 184

    4.14 Mass Spectral Detectors (MS) 185

    4.15 Other Hyphenated Detectors 191

    4.16 Sample Derivatization and Reaction Detectors 194

    References 196

    5 The Column 199

    5.1 Introduction 200

    5.2 Column Supports 200

    5.3 Stationary Phases 217

    5.4 Column Selectivity 227

     

    5.5 Column Hardware 238

    5.6 Column-Packing Methods 240

    5.7 Column Specifications 244

    5.8 Column Handling 246

    References 250

    6 Reversed-phase Chromatography for Neutral Sam- Ples 253

    6.1 Introduction 254

    6.2 Retention 256

    6.3 Selectivity 263

    6.4 Method Development and Strategies for Optimizing Selectivity 284

    6.5 Nonaqueous Reversed-Phase Chromatography (narp) 295

    6.6 Special Problems 297

    References 298

    7 Ionic Samples: Reversed-phase Ion-pair and Ion- Exchange Chromatography 303

    7.1 Introduction 304

    7.2 Acid-Base Equilibria and Reversed-Phase Retention 304

    7.3 Separation of Ionic Samples by Reversed-Phase Chromatography (RPC) 319

    7.4 Ion-Pair Chromatography (IPC) 331

    7.5 Ion-Exchange Chromatography (IEC) 349

    References 357

    8 Normal-phase Chromatography 361

    8.1 Introduction 362

    8.2 Retention 363

    8.3 Selectivity 376

    8.4 Method-Development Summary 385

    8.5 Problems in the Use of NPC 392

    8.6 Hydrophilic Interaction Chromatography (HILIC) 395

    References 401

    9 Gradient Elution 403

    9.1 Introduction 404

    9.2 Experimental Conditions and Their Effects on Separation 412

    9.3 Method Development 434

    9.4 Large-Molecule Separations 464

    9.5 Other Separation Modes 465

    9.6 Problems 470

    References 471

    10 Computer-assisted Method Development 475

    10.1 Introduction 475

    10.2 Computer-Simulation Software 481

    10.3 Other Method-Development Software 491

    10.4 Computer Simulation and Method Development 492

    References 497

    11 Qualitative and Quantitative Analysis 499

    11.1 Introduction 499

    11.2 Signal Measurement 500

    11.3 Qualitative Analysis 516

    11.4 Quantitative Analysis 520

    11.5 Summary 529

    References 529

    12 Method Validation 531
    with Michael Swartz

    12.1 Introduction 532

    12.2 Terms and Definitions 534

    12.3 System Suitability 542

    12.4 Documentation 543

    12.5 Validation for Different Pharmaceutical-Method Types 546

    12.6 Bioanalytical Methods 548

    12.7 Analytical Method Transfer (AMT) 554

    12.8 Method Adjustment or Method Modification 561

    12.9 Quality Control and Quality Assurance 564

    12.10 Summary 565

    References 566

    13 Biochemical and Synthetic Polymer Separations 569
    with Timothy Wehr, Carl Scandella, and Peter Schoenmakers

    13.1 Biomacromolecules 570

    13.2 Molecular Structure and Conformation 571

    13.3 Special Considerations for Biomolecule HPLC 579

    13.4 Separation of Peptides and Proteins 584

    13.5 Separation of Nucleic Acids 618

    13.6 Separation of Carbohydrates 625

    13.7 Separation of Viruses 630

    13.8 Size-Exclusion Chromatography (SEC) 631

    13.9 Large-Scale Purification of Large Biomolecules 641

    13.10 Synthetic Polymers 648

    References 658

    14 Enantiomer Separations 665
    with Michael Lämmerhofer, Norbert M. Maier and Wolfgang Lindner

    14.1 Introduction 666

    14.2 Background and Definitions 666

    14.3 Indirect Method 670

    14.4 Direct Method 675

    14.5 Peak Dispersion and Tailing 681

    14.6 Chiral Stationary Phases and Their Characteristics 681

    14.7 Thermodynamic Considerations 715

    References 718

    15 Preparative Separations 725
    with Geoff Cox

    15.1 Introduction 726

    15.2 Equipment for Prep-LC Separation 730

    15.3 Isocratic Elution 736

    15.4 Severely Overloaded Separation 748

    15.5 Gradient Elution 751

    15.6 Production-Scale Separation 754

    References 755

    16 Sample Preparation 757
    with Ronald Majors 757

    16.1 Introduction 758

    16.2 Types of Samples 759

    16.3 Preliminary Processing of Solid and Semi-Solid Samples 760

    16.4 Sample Preparation for Liquid Samples 764

    16.5 Liquid-Liquid Extraction 764

    16.6 Solid-Phase Extraction (SPE) 771

    16.7 Membrane Techniques in Sample Preparation 790

    16.8 Sample Preparation Methods for Solid Samples 791

    16.9 Column-Switching 796

    16.10 Sample Preparation for Biochromatography 797

    16.11 Sample Preparation for LC-MS 800

    16.12 Derivatization in HPLC 802

    References 805

    17 Troubleshooting 809

    Quick Fix 809

    17.1 Introduction 810

    17.2 Prevention of Problems 811

    17.3 Problem-Isolation Strategies 819

    17.4 Common Symptoms of HPLC Problems 821

    17.5 Troubleshooting Tables 865

    References 876

    Appendix I. Properties of HPLC Solvents 879

    I.1 Solvent-Detector Compatibility 879

    I. 2 Solvent Polarity and Selectivity 882

    I. 3 Solvent Safety 885

    References 886

    Appendix II. Preparing Buffered Mobile Phases 887

    II.1 Sequence of Operations 887

    II.2 Recipes for Some Commonly Used Buffers 888

    Reference 890

    Index 891