• Produktbild: Molecular Diagnosis of Infectious Diseases
  • Produktbild: Molecular Diagnosis of Infectious Diseases
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Molecular Diagnosis of Infectious Diseases

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

01.12.2003

Herausgeber

Jochen Decker + weitere

Verlag

Humana Press

Seitenzahl

464

Maße (L/B/H)

24,1/16/3,1 cm

Gewicht

2 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-1-58829-221-6

Beschreibung

Rezension

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics. Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. "-Doody's Health Sciences Book Review Journal

"...Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book."
-Archives of Pathology and Laboratory Medicine

"...a valuable methodological book for molecular biologists in a hospital laboratory or in academia." -Trends in Parasitology

"...a powerful tool for developing better experiments in the diagnostics of infectious diseases." -International Microbiology

"...a very good compilation of techniques that can be used for numerous applications."
-Analytical Biochemistry

"...a useful reference source for anyone who wants to manipulate microbial protein for diagnostic purposes."
-International Journal of Medical Microbiology

"...this book continues the well-established format of this series from Humana Press in providing clearly described laboratory protocols with useful background discussion...recommended as a useful laboratory reference book..." - Proteomics

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics." -Doody's Health Sciences and Book Review Journal

"...well-organized...provides detailed methods andpractical tips...the book can be recommended for students, graduate students, and postdoctoral, fellows in the field of proteomics..." - Clinical Infectious Diseases

"...brings together the key elements of oral medicine and nutrition in an accessible form for the dental professional...an excellent reference text...wholeheartedly recommend(ed)." - American Society for Clinical Nutrition

Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source." -Archives of Pathology and Laboratory Medicine

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

01.12.2003

Herausgeber

Verlag

Humana Press

Seitenzahl

464

Maße (L/B/H)

24,1/16/3,1 cm

Gewicht

2 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-1-58829-221-6

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Molecular Diagnosis of Infectious Diseases
  • Produktbild: Molecular Diagnosis of Infectious Diseases
  • Part I. Protein-Based Identification of Antigens

    Proteomic Approaches to Antigen Discovery
    Karen M. Dobos, John S. Spencer, Ian M. Orme, and John T. Belisle

    Immunoproteomics
    Alexander Krah and Peter R. Jungblut

    Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
    Stephen Thompson

    Part II. DNA-Based Identification of Antigens

    Representational Difference Analysis of cDNA
    Lucas D. Bowler

    Microarray Data Analysis and Mining
    Silvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. Calogero

    Expression Cloning
    Michael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A. W. Skeiky

    Part III. Identification of Epitopes and Immunomodulatory Components

    Determination of Epitopes by Mass Spectrometry
    Christine Hager-Braun and Kenneth B. Tomer

    Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
    Timothy W. Tobery and Michael J. Caulfield

    Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
    Ludwig Deml, Jens Wild, and Ralf Wagner

    Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
    Ludwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-Brachert

    Part IV. Expression of Recombinant Proteins

    High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
    Jutta Drees, Jason Smith, Frank Schäfer, and Kerstin Steinert

    Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
    Markus Fuhrmann

    Codon-Optimized Genes that Enable Increased Heterologous Expression inMammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
    Marcus Graf, Ludwig Deml, and Ralf Wagner

    Part V. Purification, Modification, and Renaturation of Recombinant Proteins

    Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
    Udo Reischl

    Purification of Recombinant Proteins with High Isoelectric Points
    Raffaele A. Calogero and Anna Aulicino

    Refolding of Inclusion Body Proteins
    Marcus Mayer and Johannes Buchner

    Small-Molecule-Protein Conjugation Procedures
    Stephen Thompson

    Part VI. Characterization of Recombinant Proteins

    Structural Characterization of Proteins and Peptides
    Rainer Deutzmann

    Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
    Claudia Hahnefeld, Stephan Drewianka, and Friedrich W. Herberg

    Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
    Uwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans Wolf

    Part VII. Evaluation of Recombinant Proteins in Immunological Test Systems

    Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
    John E. Butler

    Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
    Alan Warnes, Anthony R. Fooks, and John R. Stephenson

    Basic Problems of Serological Laboratory Diagnosis
    Walter Fierz

    Molecular Diagnostics Resources on the Internet
    Larry Winger

    Part VIII. Recombinant Receptor Molecules

    Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
    Lars Toleikis, Olaf Broders, and Stefan Dübel

    Index