• Produktbild: RNA Isolation and Characterization Protocols
  • Produktbild: RNA Isolation and Characterization Protocols
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RNA Isolation and Characterization Protocols

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

08.04.1998

Abbildungen

XIII, 264 p.

Herausgeber

Ralph Rapley + weitere

Verlag

Humana Press

Seitenzahl

264

Maße (L/B/H)

23,5/15,7/2 cm

Gewicht

568 g

Auflage

1998

Sprache

Englisch

ISBN

978-0-89603-494-5

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

08.04.1998

Abbildungen

XIII, 264 p.

Herausgeber

Verlag

Humana Press

Seitenzahl

264

Maße (L/B/H)

23,5/15,7/2 cm

Gewicht

568 g

Auflage

1998

Sprache

Englisch

ISBN

978-0-89603-494-5

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: RNA Isolation and Characterization Protocols
  • Produktbild: RNA Isolation and Characterization Protocols
  • to Isolating RNA.- Large and Small Scale RNA Preparations from Eukaryotic Cells.- An Improved Rapid Method of Isolating RNA from Cultured Cells.- Isolating RNA with the Cationic Surfactant, Catrimox-14.- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues.- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides.- Isolation of Plant Mitochondrial RNA from Green Leaves.- Extraction of RNA from Fresh and Frozen Blood.- Isolation of Total RNA from Bacteria.- Isolation of Total RNA from Tissues or Cell Lines.- Isolation of Messenger RNA.- UV Spectrophotometric Analysis of Ribonucleic Acids.- Formaldehyde Gel Electrophoresis of Total RNA.- Preparation of RNA Dot-Blots.- Nonradioactive Northern Blotting.- The Use of RNA Probes for the Analysis of Gene Expression.- Analysis of RNA by Northern Blotting Using Riboprobes.- RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues.- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing.- Preparation of Tissue Sections and Slides for mRNA Hybridization.- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes.- One-Tube RT-PCR with Sequence-Specific Primers.- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA.- Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA.- Gene Expression Analysis by CD-RT-PCR Eric de Kant.- Primer Extension Analysis of mRNA.- S1 Mapping Using Single-Stranded DNA Probes.- Measurements of Rate of Transcription in Isolated Nuclie by Nuclear “Run-Off” Assay.- Transcription In Vitro Using Bacteriophage RNA Polymerases.- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System.- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System.- The Xenopus Egg Extract Translation System.- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization.